| Pores formation on cell membranes by hederacolchiside A1 leads to a rapid release of proteins for cytosolic sub-proteome analysis |
|
|
|
|
G.D. Mazzucchelli, N.A. Cellier, V. Mshviladzade, R. Elias, Y.-H. Shim, D. Touboul, L. Quinton, A. Brunelle, O. Laprévote, E.A. De Pauw, M. -C. De Pauw-Gillet J. Proteome Res. 2008, 7, 1683-1692. Hederacolchiside A1 was used to progressively permeabilize the membrane of human melanoma MEL-5 cells. Holes formation was followed by Scanning Electron Microscopy and interaction of the saponin with cholesterol and phospholipids by TOF-SIMS. 2D-LC-MS/MS and 2D-SDS-PAGE show that the release of soluble proteins into serum-free culture media increases with time. This can lead to a new rapid and efficient strategy to analyze the cytosolic subproteome and it opens the door to get information from the cytosolic compartment for clinical proteomic studies. |
| < Prev | Next > |
|---|